Process for fermenting rapamycin with high yield

The invention provides a process for fermenting rapamycin with high yield, and belongs to a method for preparing antibiotics by utilizing fermentation of microorganism in the technical field of biology. According to the method, enzymolytic cotton seed powder, dextrin, glycerinum, yeast extract, L-lysine, KH2PO4, K2HPO4, (NH4)2SO4 and trace elements as culture media, wherein the trace elements contain Co , glycerinum and phosphate buffer are fed in the early phase of the fermenting culture process, and L-pipecolic acid and shikimic acid are fed in the synthesizing phase. By adopting the method, the methyl derivative can be reduced in the growing phase, and the content of rapamycin is increased; intermediate products can be reduced, and rapamycin can generate key enzyme activity; the beneficial effect of high yield of rapamycin can be achieved.