Cytokine-Induced Neutrophil Chemoattractant (CINC)-2 α, a Novel Member of Rat GRO/CINCs, Is a Predominant Chemokine Produced by Lipopolysaccharide-Stimulated Rat Macrophages in Culture☆

Abstract Neutrophil chemotactic factors (chemokines) have been purified from conditioned medium of lipopolysaccharide (LPS)-stimulated rat macrophages in culture. The LPS-stimulated macrophages produced one acidic chemokine, rat macrophage inflammatory protein (MIP)-1α , and four basic chemokines, cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2α , CINC-2β and CINC-3/rat MIP-2. CINC-2α , a novel chemokine recently isolated from conditioned medium of rat granulation-tissue culture, was the major chemoattractant among these four basic chemokines. The results suggest that CINC-2α is produced by activated macrophages in vivo and plays an important role in the infiltration of neutrophils into inflammatory sites in rats.