Inheritance and mapping for resistant genes of soybean adult-plant and seed coat mottling to soybean mosaic virus no 3 strain

SMV3 is one of the SMV strains of northeast China, it has the strongest pathogenicity and can cause serious consequences when it occurs around the large planning area. The objective of this study was to evaluate the genetic regularity of resistance to SMV3 and to map the resistant genes associated with SSR markers for soybean adult-plant and seed coat mottling. To F2 populations, the parents and the relative F2:3 populations were used to evaluate the genetic regularity underlying SMV3 resistance. One F2 population (288 individuals plants) was constructed by susceptible cultivar 3C624 crossed with resistant cultivar DongNong 8143, another F2 population (300 individual plants) was constructed by resistant cultivar DongNong 8143 crossed with susceptible cultivar 3C624. 1000 pairs of SSR molecular markers and genetic analytic software were used to map the resistant genes, 11 SSR markers associated with the resistant genes were mapped successfully. The result indicated that adult-plant resistance to SMV3 of DongNong 8143 was controlled by one single dominant nuclear gene; seed coat mottling resistance to SMV3 were controlled by two genes which were inherited dependent to each other; the resistance of adult-plant and seed coat mottling to SMV3 also inherited jointly, the linkage distance was 22.07cM and 15.79cM, respectively. Two resistant genes of seed coat mottling RSMV3-S1, RSMV3-S2 were named by using Gene Interaction V1.0, they were mapped on the linkage group F and associated with SSR markers. The order and linkage distance between SSR markers and two resistant genes were Satt030-10.1cM-RSMV-S1-11.5cM-Sat_240, Satt114-6.5cM- RSMV-S2-75cM-Satt335. This research provides the useful information for breeders to select the two types of SMV3 resistance simultaneously in soybean breeding through molecular marker assisted selection (MAS).