Preparation of National Reference Serum Used for Detection Kit for IgG against Toxoplasma

Objective To prepare national reference serum used for detection kit for IgG against toxoplasma.Methods The sera positive for IgG against toxoplasma as well as normal human sera were collected and screened by using detection kits manufactured by six manufacturers and confirmed by dye test and direct agglutination test,then the positive serum samples were calibrated with international standard for IgG against toxoplasma.The references were calibrated coordinately,based on which the minimum detection limit was determined.Results A set of national reference consisting of 9 positive(including one for minimum detection limit) and 15 negative serum samples was prepared.Conclusion The first set of national reference serum used for detection kit for IgG against toxoplasma was prepared,and the corresponding quality standard was developed,which has been approved by the relevant national departments.