Prediction of embryo viability using validated cell division time intervals measured by time-lapse imaging

formed. A ZILOS-tk laser was used during the TE biopsy procedure. Embryos were cultured overnight under low O2 in 10%SPS Blast Media (Sage). Approx. 28 hours post thaw, embryos were observed again for survivability and transferability. RESULTS: 18 patients consented to have previously vitrified blasts donated for in-house research. 53 blasts survived warming and were biopsied after four hours. Biopsied blasts were observed the following day and graded using Gardner’s grading system. A grade of 3CC was used as the lower threshold for survival and transfer suitability. Based on this criteria, 49 blasts biopsied appeared suitable for transfer. CONCLUSION: This study demonstrates high competency of blasts vitrified, thawed, biopsied and cultured overnight. This is useful information for clinics and patients that have undiagnosed, vitrified blasts in storage and would now like to return and apply PGS testing during an FET cycle. Provided PGS testing can be accurately performed overnight, euploid blasts can be identified and transferred. With enhanced PGS turnaround testing time, this is a unique, novel approach that has promising future potential.