Characterization of an indigenous isolate of cyst nematode specific Pasteuria isolate

An isolate of Heterodera cajani Pasteuria (HcP) was characterized for its genetic lineage and host specificity. The 16S rDNA characterization of the isolate showed 98% identity with another HcP isolate (Accession No. JN 592479) and 97% identity with P. nishizawae isolates. In the host range studies, an average of 14, 2.5 and 13.6 spores adhered to the J2 of H. cajani, H. avenae and G. pallida respectively. However, there was no spore attachment observed on Rotylenchulus reniformis, Meloidogyne incognita, Meloidogyne graminicola, Pratylenchus spp., Hoplolaimus spp., Heterorhabditis indica, Steinernema glaseri and S. abbasi. After attachment to the J2 the isolate completed its life cycle in H. cajani in 26 days but not in H. avenae.