Maternal-effect Floped gene is essential for the derivation of embryonic stem cells in mice
2013
Floped (official name Ooep) is specifically and abundantly expressed in
mouse oocytes and embryonic stem cells (ESCs). Depletion of Floped from
oocytes leads to early embryonic arrest at the 2-cell stage. Although
crucial in cleavage stage development, its roles in early embryos as
well as in ESCs remain completely unknown. Here, we compared the
efficiency of mouse ESC derivation from inner cell mass (ICM) with and
without Floped to study its possible roles in mESCs. Derivation rates
of mESC from wild-type, heterozygous, and homozygous blastocysts were
33.3%, 21.43%, and 3.85%, respectively, indicating that Floped-/-
blastocysts had significantly decreased derivation rates. Respective
outgrowth appearing rate five days after blastocyst attachment were
83.3%, 85.7%, and 15.4%. Morphologically, the outgrowth of ICM from
Floped-/- blastocysts appeared severely death three to five days after
blastocyst attachment, and the respective derived stem cells showed
long-term instability with long-standing epithelial-like colonies. This
result suggests a possible role of Floped in the course of ICM-ESCs
transition.
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