Pelabelan Antibodianti-ns1 Dengue Kelinci dengan Horseradish Peroksidase

Dengue NSI protein can be an ideal target for early detection of dengue virus infection. The aim of the study is to label rabbit anti-NSI antibody with HRP, so that it can be used for detection of NSI protein. The design of this study is laboratory experimental. Anti-NSI IgG-contained rabbit serum was purified with column chromatography (Sephadex G-200). The result of purification was labeled with HRP using periodate method. Then, HRP-labeled IgG was generated with dot blot and ELISA. Using dot blot assay, we found that rabbit anti-NSI IgG labeled with HRP is successful. Nevertheless, the ability of detection was not so good (1:1600). In addition, HRP-labeled IgG used to detect NSI protein utilizing ELISA resulted in high negative control absorbance (0,453 ± 0,013). Therefore, we cannot interpret the assay. The labeling was successful, but it need further optimatization in order to get the HRP-labeled IgG can be used in ELISA. Optimatization was also needed to increasing the ability of detection of HRP-labeled IgG in dot blot assay. Key words: protein NSI, dengue anti-NSI IgG, labeling, HRP