Analysis of the Detection Efficiency of Digoxin Labeling at 3′ End

[Objective] The aim of the research was to provide basis for nucleic acid hybridization test.[Method] Three probes of nucleic acid with different length were used to label at 3′ end with Digoxin and detect its labeling efficiency.The effects of the length and concenstration of probes and its fixation methods on nylon membrane on the signal intensity were studied.[Result] The results of polyacrylamide gel electrophoresis showed that the probes with Digoxin labeling was hysteresis than that without labeling,which indicated that the labeling was more successful.The detected signal intensity was reduced with the reduction of concenstration of probe.The fixation method of probe also had certain effects on the signal intensity.Both ultraviolet cross-linking and high temperature firing could make the detection signal be strengthened.And the signal intensity at ultraviolet cross-linking was higher.[Conclusion] The detected signal intensity of Digoxin was related with the length,concentration,fixation and the combined efficacy between nuceleic acid and membrane.