Analgesic and Anti-Inflammatory Activity ofEthyl Acetate Extract of Hibiscuscannabinus (L.) Seed Extract

Background and Objectives: Hibiscus cannabinus L. is a well known herbaceous annual plant which has been used in traditional medicine for the treatment of hepatotoxicity and various inflammatory diseases. Therefore, present study was planned to evaluate the antinociceptive and anti-inflammatory activity of Hibiscus cannabinus (L.) using several acute and chronic models of pain in mice and rats. Methods: Antinociceptive activity was done using Writhing test, tail immersion and hot plate test while anti-inflammatory activity was carried out with carrageenan, serotonin and histamine induced rat paw edema in rat models. Oral administration of 100, 200 and 400 mg/kg of seed extracts of ethyl acetate exctract of Hibiscus cannabinus were used for the above study. Results: The seed extract at 100, 200 and 400 showed significant reduction in acetic acid induced writhings in mice with maximum effect at 400 mg/kg dose. In tail immersion and hot plate method, significant effect were observed at a doses of 200 and 400 mg/kg of EAHC extract. The extract also significantly decreased the rat paw edema volume induced by carrageenan, serotonin and histamine at a dose 400 mg/kg of EAHC extract. Conclusion: Results obtained from the present study demonstrated that ethyl acetate extract of hibiscus cannabinus seed has central and peripheral analgesic as well as anti-inflammatory activities.