Enzyme I facilitates reverse flux from pyruvate to phosphoenolpyruvate in Escherichia coli.

Enzyme I, a component of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS), converts phosphoenolpyruvate to pyruvate. Here, the authors show that Enzyme I facilitates also the reverse reaction during both gluconeogenic and glycolytic growth in E. coli.