A bacterial genetic selection system for ubiquitylation cascade discovery

Olga Levin-Kravets,Neta Tanner,Noa Shohat,Ilan Attali,Tal Keren-Kaplan,Anna Shusterman,Shay Artzi,Alexander Varvak,Yael Reshef,Xiaojing Shi,Ori Zucker,Tamir Baram,Corine Katina,Inbar Pilzer,Shay Ben-Aroya,Gali Prag

A bacterial genetic selection system for ubiquitylation cascade discovery

2016

Olga Levin-Kravets
Neta Tanner
Noa Shohat
Ilan Attali
Tal Keren-Kaplan
Anna Shusterman
Shay Artzi
Alexander Varvak
Yael Reshef
Xiaojing Shi
Ori Zucker
Tamir Baram
Corine Katina
Inbar Pilzer
Shay Ben-Aroya
Gali Prag

An Escherichia coli-based genetic selection system, constructed from a split antibiotic resistance protein individually tethered to ubiquitin and a target protein, helps discover ubiquitin cascade pathway interactions.

Keywords:

Pathway (interactions)
Target protein
Enzyme
Molecular biology
Cascade
Escherichia coli
Ubiquitin
Bioinformatics
Antibiotic resistance
High-throughput screening
Genetics
Biology
genetic interaction
genetic selection

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