Exercise ameliorates insulin resistance and improves SIRT6-mediated insulin signaling transduction in liver of obese rats

Physical exercise is essential for the amelioration of insulin resistance. The mechanisms in charge of improved insulin resistance, regulated by exercise, are insufficient inquired. Previous researches revealed that SIRT6-mediated insulin signaling acts a crucial character in hepatic IR. The objective of our research was to inquire the effects of exercise on SIRT6-mediated insulin signaling in liver of IR rats. Forty male Sprague-Dawley rats were randomly assigned to four groups (n=10 rats each): control rats fed with standard chow (Lean group); sedentary rats fed with HFD (HFD-SED); rats fed with HFD and submitted to 8-week chronic swimming exercise training (HFD-CE) and submitted to one-off acute swimming exercise training (HFD-AE). HFD feeding leaded to increased body weight, hepatic TG accumulation and serum FFA, and enhanced gluconeogenesis. Besides, HFD feeding decreased body insulin sensitivity. Hepatic USP10 and SIRT6 protein levels decreased under obese status. Exercise intervention, both chronic and acute exercise intervention alleviated physiological and metabolic status, increased hepatic USP10 and SIRT6 levels, improved insulin signaling transduction and inhibited gluconeogenesis. These consequences disclosed that exercise intervention produced a regulation in SIRT6-mediated insulin signaling, which afford significant progress in realization of the latent molecular mechanism, which concatenated exercise intervention to a mitigation of IR。.