An Experimental Study on Effect of Chemotherapeutic Agents Combined with Nimotuzumab on Human Colon Cell Line LoVo

Objective To investigate the effect and the possible mechanisms of cytotoxic agents combined with nimotuzumab in human colon LoVo cell line.Methods Inhibition of cell proliferation was evaluated by MTT assay.The combination effect was determined by q value.Cell cycle and apoptosis were analyzed by FACS.Results Combined treatment with h-R3 and nimotuzumab could not only enhance the growth inhibition but also increase apoptosis and cell cycle(G0 /G1 and G2 /M) arrest of LoVo cells.The effect was less apparent when h-R3 was combined with 5-FU.Enhancing effect was not observed when h-R3 was combined with L-OHP.Conclusion Nimotuzumab could enhance the therapeutic actions of certain chemotherapeutic agents on LoVo cells.