Determination of calcium, magnesium, phosphorus, iron, and zinc in buffalo milk by inductively coupled plasma-atomic emission spectrometry (ICP-AES).

Objective To establish a method for determination of calcium, magnesium, phosphorus, iron and zinc in buffalo milk by inductively coupled plasma-atomic emission spectrometry(ICP-AES) with microwave sample digestion. Methods The buffalo milk samples were dissolved by microwave digestion using nitric acid and hydrogen peroxide as solvent and determined by inductively coupled plasma-atomic emission spectrometry (ICP-AES). The spectral lines for Ca, Mg, P, Fe and Zn were 317.993 nm{106}, 202.582 nm{466}, 178.284 nm{489}, 238.204 nm{141} and 213.856 nm{457}. Results The detection limit of Ca, Mg, P, Fe and Zn were 0.091, 0.063, 0.037, 0.019 mg/kg and 0.012 mg/kg. The recoveries for these elements were 94.7%∼108.2% with relative standard deviation(RSD, n=11) less than 2.5%. Conclusion The analytical results consisted with those obtained by national standard method. This method has many advantages, such as good repeatability, less use of digestion reagent, easy operation, synchronous determination of multiple elements, fast testing velocity, and less pollution. 第 4期 许 光, 等: ICP-AES法同时测定水牛奶中钙、镁、磷、铁和锌 1147