Comparison of Protein Components of Excretory-Secretory Antigens of Adult Worm and Muscle Larvae of Trichinella spiralis

Objective To compare the protein components of excretory-secretory(ES)antigens of adult worm and muscle larvae of Trichinella spiralis,analyze their reactogenicities and lay a foundation of separation and screening of Tirchinella spiralis antigen component with high immunogenicity and reactogenicity.Methods The adult worms and muscle larvae of Tirchinella spiralis were collected,with which ES antigens were prepared then analyzed for protein components by SDS-PAGE and for reactogenicities by Western blot.Results Seventeen protein bands with relative molecular masses of 120 000 ～ 14 000,including six main protein bands with relative molecular masses of 120 000,64 000,43 000,40 000,35 000 and 33 000 respectively were observed on the SDS-PAGE profile of adult worm of Trichinella spiralis.However,twenty protein bands with relative molecular masses of 112 000 ～ 10 000,including eleven main bands with relative molecular masses of 112 000,66 000,56 000,55 000,53 000,49 000,45 000,43 000,25 000,21 000 and 10 000 respectively were observed on the SDS-PAGE profile of muscle larvae.The ES antigen of adult worm showed seven reaction bands on Western blot profile,with relative molecular masses of 43 000,40 000,35 000,27 000,19 000,18 000 and 14 000 respectively,of which the ones with relative molecular masses of 43 000,40 000,27 000 and 18 000 were stained obviously.However,the ES antigen of muscle larvae showed fourteen bands on Western blot profile,with relative molecular masses of 74 000 ～ 12 000,of which the ones with relative molecular masses of 53 000,49 000,45 000,43 000,35 000,27 000,18 000 and 12 000 were stained obviously.Conclusion Both the protein components of adult worm and muscle larvae of Trichinella spiralis were complicated,including common and different components.ES antigen showed high reactogencity and might be used as an important candidate antigen for study on trichinelliasis.