Acceptor Specificities of α-Mannosidases from Jack Bean and Almond, and Trans-mannosylation of Branched Cyclodextrins

Jack bean α-mannosidase had a wide acceptor specificity and could transfer mannosyl residues to various acceptors such as d-fructose, l-arabinose, maltose, lactose, and sucrose. The structures of the transferred products of branchd cyclodextrins (CDs) (glucosyl-βCD, maltosyl-αCD, and maltosyl-βCD) were found to be α-d-mannosyl-(1→6)-α-d-glucosyl-(1→6)-βCD, α-d-mannosyl-(1→6)-α-d-glucosyl-(1→4)-α-d-glucosyl-(1→6)-αCD and α-d-mannosyl-(1→6)-α-d-glucosyl-(1→4)-α-d-glucosyl-(1→6)-βCD, respectively. Almond α-mannosidase also produced the same transmannosylated products of branched CDs.