Effects of imatinib on differentiation and function of dendritic cells generated from CD34~+ peripheral blood progenitor cells in vitro

Aim: To study the effects of imatinib on the differentiation and function of dendritic cells(DCs) generated from CD34+ peripheral blood progenitor cells in vitro.Methods: The isolated PBMNCs were allocated into control group and imatinib groups with different doses (1 μmol/L,3 μmol/L, 5 μmol/L). The PBMNCs in each group were induced by cytokines to obtain DCs. Effects of imatinib on the differentiation and function of DCs were analyzed by flow cytometry and mixed lymphocyte reaction. Results: DCs obtained from culture in the presence of imatinib showed concentration-dependent reduced expression of CD1a, CD83 and costimulatory molecules such as CD80 and CD86. Furthermore, imatinib inhibited the induction of primary cytotoxic T-lymphocyte responses. Conclusion: Imatinib inhibits the differentiation and function of DCs that originated from CD34+ progenitor cells in vitro.