Study of AMPK signal transduction mediated the expression of adiponectin in 3T3-L1 adipocytes increased by CTRP3

Objective To investigate the role of AMP activated protein kinase (AMPK)signal in the modulation of Clq/TNF related protein 3 (CTRP3) on the expression of adiponectin in adipocytes.Methods The 3T3-L1 adipocytes were divided into control group,CTRP3 (250 μg/L) intervention group and CTRP3 (250 μg/L)+ Compound C (10 μmol/L pretreated for 1 h) intervention group.The content of adiponectin in supernatant was detected by enzyme linked immunosorbent assay.The relative expression of adiponectin mRNA was detected by real-time polymerase chain reaction.The relative expression of AMPK (thr172) was detected by Western blot.Results Compared with control group,expression of adiponectin mRNA and protein increased 53.0％ (q =15.09,P＜0.01) and 63.3％ (q =8.11,P＜0.01) respectively,the expression of AMPK(thr172) increased 43.0％ (q =14.64,P＜0.01) in CTRP3 intervention group.Compared with CTRP3 intervention group,the expression of AMPK(thr172) in CTRP3+ Compound C intervention group decreased 57.3％ (q =27.92,P＜0.01),expression of adiponectin mRNA and protein decreased 26.1％ (q =11.39,P＜0.01) and 54.1％ (q =11.31,P＜0.01) respectively.Conclusion AMPK signal participates in the modulation of CTRP3 on the expression of adiponectin in 3T3-L1 adipocytes. Key words: CTRP3 ; 3T3-L1 adipocytes; Adiponectin; AMPK