Rapid intraoperative margin assessment by using multi-modal third-harmonic generation and three-photon fluorescence microscopy

Slide-free imaging of hematoxylin-eosin-stained whole mount tissues is demonstrated using multi-modal third harmonic generation and three photon fluorescence microscopy. As a result, the demonstrated microscopy shows promise for rapid margin assessment during surgery without compromising the contrast and features of conventional H&E histology.