Substrate Influence on Mycotoxin Production of Fusarium Species and their Analytical Detection

Several strains of Fusarium culmorum, F. graminearum, F. moniliforme, F. tricinctum, F. rosevm, F. semitectum and F. sporotrichioides were investigated on corn and rice substrate for F-2, zearalenol and T-2 toxin synthesis. Toxin production was confirmed by GLC and estimated by TCLC. Substrate didn’t influence significantly recovery of the investigated mycotoxins in case of rice and corn and was about 70%. Sensitivity of TLC method is lowest for T-2 (0,05 ng/spot), detection of F-2 and zearalenol could be improved with Sarudi reagent Toxin synthesis of individual strains showed depence on substrate.