Antioxidation of polysaccharides from PERICARPIUM CITRI RETICULATAE.

[Objective] To study on the antioxidation of polysaccharides from PERICARPIUM CITRI RETICULATAE.[Method] Isolation and purification of polysaccharides from PERICARPIUM CITRI RETICULATAE were conducted by hot water extraction method.Extraction rate of polysaccharides from PERICARPIUM CITRI RETICULATAE was determined by phenol-sulfuric acid method.Clearance effect of ·OH was studied by salicylic acid method.[Result] Polysaccharides content in PERICARPIUM CITRI RETICULATAE was 6.32%;polysaccharides from PERICARPIUM CITRI RETICULATAE at different concentrations had clearance effect on ·OH,which was produced by Fenton method.With the increase of concentration,the clearance rate gradually enhanced.In other words,there was certain dose-effect relationship between clearance rate and polysaccharides dosage.[Conclusion] Polysaccharides in PERICARPIUM CITRI RETICULATAE had significant antioxidation and had clearance effect on ·OH.