Dextranase および Monofluorophosphate 配合歯磨剤のウサギおよびハムスターに対する局所刺激性について

Experiment was done as to irritation on rabbit skin, eye and cheek pouch of hamster by applying three different types of toothpaste;1. Toothpaste which was mixed Dextranase 10, 000u/g & Monofluorophosphate 1, 000p.p.m(F), 2. Toothpaste which was only contained Monofluorophosphate, 3. And which was contained none of each.1. Evaluation score by Draize Method was ranged between 0.5-0.67 of each toothpaste as to irritation on the rabbit skin.Same evaluation score was ranged between 0.83-1.00 as to pinching method to the rabbit skin by means of injection needle, inflammation by which was induced disappeared within 72 hours.2. No responce was observed as to either cornea or iris on the irritation to the eye due to Draize Method from each of three types of toothpaste.Only slight responce was, however, noticed on the conjunctiva, which disappeared within 72 hours.3. No abnormality was observed as to either optic or histological observation on mucous membrane, connective tissue beneath, and muscle layer of hamster cheek pouch by trree types of toothpastes.Conclusion was drawn that irritation induced on skin, eye and oral membrane was extremely slight by toothpaste either of which mixed Dextranase 10, 000u/g or Monofluorophosphate 1, 000p.p.m.It was not observed accebrated effect on irritation out of toothpaste which was even mixed both components up, which stayed within the range of irritation of ordinal toothpaste.It was also obrerved that ordinall toothpaste had negligible irritation in contrast to the standard level of Draize Method.