Analysis of Genetic Diversity and Relationship of Half-sib Tea Cultivars Related to Fuding Dabai and Yunnan Daye Using EST-SSR Markers

40 half-sib tea cultivars related to Fuding Dabai and Yunnan Daye were used to analyze the genetic diversity and relationship based on EST-SSR markers. Totally 99 alleles were amplified using the 28 pairs of EST-SSR primers, the number of alleles per primer ranged from 2 to 6, on average of 3.54. The polymorphism information content (PIC) varied from 0.13 to 0.79, on average of 0.59. The expected heterozygosity (He) varied from 0.08 to 0.84 and the observed heterozygosity (Ho) ranged from 0.08 to 1.00, on average of 0.59 and 0.71, respectively. The Shannon index of the population was 1.14. The 40 half-sib tea cultivars were classified into 4 groups with the similarity coefficient at 0.55. The results showed that the genetic polymorphism of half-sib tea cultivars related to Fuding Dabai was more abundant than those of Yunnan Daye.