The Development of Rs2853826 Genotyping and the Initial Association with Breast Cancer Risk in Vietnamese Women

Breast cancer (BC) is known as the leading cause of dead in women through worldwide including Vietnam. Recently, mitochondrial genome mutations concerned to breast cancer has been screened in order to propose new potential biomarkers. The SNP rs2853826 located at 10398-nucleotide position within the NADH-dehydrogenase3 has been demonstrated to be associated with BC in populations such as Malaysian, Northern Indian, Poland, Banglasdesh, African-American, Eropean-American, but Vietnam. Thus, rs2853826 was selected as the candidate SNP for investigation in Vietnamese population. In this study, the High Resolution Melting method is optimized for genotyping 100/100 cases/controls samples, then determined the association between this SNP and the disease. The genotyping results revealed SNP rs2853826 has high polymorphism with the minor allele frequency, G allele, was 17.2%. risk allele G was 17.2 %. The association result between rs2853826 and BC revealed that G allele tend to increases risk of BC among Vietnamese when comparing A allele (OR[95%CI]=1.224[0.818-1.832]); and homozygote and heterozygote model also showed this trend when compared to dominant model. Since the p-values were larger 0.05 (p=0.326), our results only show a trend correlation rather than a significant association between SNP rs2853826 and BC risk. However, the reliability of this association analysis result is low (7.57%), so this research needs to be conducted with a larger sample size to reach the greater power (>90%) for therelation.