Method of producing 2-hydroxy-gamma-butyrolactone

Gamma-2-hydroxy-2, according to one embodiment of the present invention method of producing butyrolactone comprising the steps of: preparing a 3-hydroxypropionic acid (3-hydroxypropionic acid); Contacting a CoA (Coenzyme A) and a first enzyme to the 3-hydroxy-propionic acid 3-hydroxy-propionyl obtaining -CoA (3-hydroxy-propionyl-CoA); Obtaining the formic acid to propionyl -CoA (formic acid) and contacting the second enzyme 4-hydroxy-2-oxo-butanoate acid (4-hydroxy-2-oxobutanoic acid) - The 3-hydroxy; To obtain the 4-hydroxyl by contact of the third enzyme in the hydroxy-2-oxo-butanoate acid 2,4-dihydroxy-butanoate acid (2,4-dihydroxy-butanoic acid); And said 2,4-dihydroxy-butanoate by contacting the fourth enzyme in acid 2-hydroxy-butyrolactone to afford a (2-hydroxy-gamma-butyrolactone) - gamma; includes. The first enzyme is hydroxy which consumes ATP - propionyl -CoA- new teote rise (3-hydroxy-propionyl-CoA synthetase), and the second enzyme is a 2-keto-butyrate formate lyase (2-ketobutyrate formate lyase), and said third enzyme is a 2-oxo-butyrate dehydrogenase (2-oxobutyrate dehydrogenase), the fourth enzyme is a para-oxo-xylanase (paraoxonase).