Determination of nevirapine in plasma by GC-MS.

Here we describe a simple, stable, and specific gas chromatography‐mass spectrometry (GC‐MS) method for the determination of nevirapine in plasma. After precipitation of proteins, the non-nucleoside reverse transcriptase inhibitor nevirapine was extracted with dichloromethane. For the determination and quantification of nevirapine, 1 µL of the organic layer was injected onto the GC‐MS system. Linear calibration curves were obtained with BIRH 0414BS as internal standard in a range from 0.01 to 15 µg/mL. Intra- and inter-day accuracy and precision of this method were good with an accuracy between 96‐109% and a precision between 2‐8% across the therapeutic range of nevirapine. GC‐MS proved to be a valid alternative to high-performance liquid chromatography and liquid chromatography‐MS.