In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation Elajik asidin siçan C6 glioma hücre kültürlerinde sitotoksisite ve proliferasyon üzerine in vitro etkileri

Objectives: Ellagic acid is a plant-derived polyphenolic compound. The aim of this study is to investigate in vitro effects of ellagic acid on the proliferation and viability of rat C6 glioma cell lineage depending on dose and time and also is to evaluate ultrastructural changes in C6 glioma spheroids. Materials and methods: Effects of ellagic acid on rat C6 glioma cell line were investigated by using two-dimensional models of tumor cell culture depending on dose and time. The effects of Ellagic acid was evaluated as cell proliferation, cell viability, and synthesis phase of cell cycle, and cell structure at 24 th , 48 th , 72 nd hours. Cell structure was evaluated at 24 th and 72 nd hours in three dimensional cell culture spheroid models. Results: Reducing effect of ellagic acid on cell proliferation and cell viability was seen in two dimensional cultures (p 0. 05). Electron micrographs in which three dimensional cell culture spheroid models was investigated, structural changes was found to be different from the control group. Conclusions: In the evaluation of effects of ellagic acid on rat C6 glioma cells using two-dimensional culture models, it was observed that ellagic acid caused reduced cell viability, deterioration in cell structure and prevented cell proliferation. J Clin Exp Invest 2012; 3 (3): 350-356