Use of the method of fast atom bombardment mass spectrometry for identification of products of the interaction of thiophosphamide with DNA

Products of interaction between DNA and an antitumour drug N, N', N''-triethylenethiophosphoramide (thiotepa) have been observed for the first time by the fast atom bombardment mass spectrometry. The sites of alkylation are detected as N7 (Gua) and N3 (Ade), and yields of the products are evaluated.