Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation–multi-angle light scattering

The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation–multi-angle light scattering (AsFlFFF-MALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 °C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-β-d-thiogalactoside (IPTG). For GFPIBs prepared at 30 °C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.