The coastal areas of Yantai human and swine hepatitis E virus genotyping analysis

Objective Survey of the coastal city of Yantai,from human and swine hepatitis E virus (HEV) genotype correlation. Method Application of reverse transcription nested polymerase chain reaction (RT - nPCR) method for local acute sporadic hepatitis E patients,normal population of HEV-IgM positive and local pig farm pigs were HEV RNA detection.And HEV RNA positive samples for cloning sequencing and sequence analysis.Results In 16 patients with acute sporadic hepatitis E in 7 cases of RNA positive stool specimens of HEV; 51 lgM positive sera of normal people in specimens with 1 HEV RNA positive; 34 pig bile specimens with 1 HEV RNA positive. Sequence analysis revealed the region HEV strains and swine strains in the ORF2 region of nucleotide sequence homology is 87％ -98.1％.7 strains of hepatitis E virus genotype in patients and 1 strains of swine hepatitis E virus genotypes are type Ⅳ,gene sequence homology between the 87％ -98.1％ ; there were 6 patients and porcine gene sequence homology in 93.9％ -98.1％ between,for type a subtype; 1 patients and porcine gene sequence homology in 87％,for the type D subtype.Normal population of 1 cases of hepatitis E virus genotype for I type D subtype.Human and porcine HEV ORF2 gene fragment and HEV part Ⅰ-Ⅳ representative strains were compared,and the nucleotide sequence homology were 82.5％ - 100％,81.7％ - 92.9％,81.4％ - 93.9％,84.9％ - 100％.Conclusion The area population prevalence of HEV in the presence of 2 genotype 3 subtype genes,mainly to Ⅳ A,in pigs with popular HEV gene with a high homology; HEV type Ⅰ in the crowd disperses in the presence of. Key words: Hepatitis E virus; Genotype; Sequence analysis; Models,animal