Efficient phosphatidylserine synthesis by a phospholipase D from Streptomyces sp. SC734 isolated from soil‐contaminated palm oil

In this contribution the synthesis of phosphatidylserine (PS) using a phospholipase D (PLD) produced by Streptomyces sp. isolated from soil- and wastewater-contaminated palm oil is shown. Streptomyces sp. SC734 was found to produce PLD in the culture medium and showed the highest transphosphatidylation activity. This PLD was purified and characterized. The purified PLDSC734 converted phosphatidylcholine (PC) to PS completely in a biphasic system consisting of chloroform and sodium acetate buffer (pH 6.0) at 1:5 (mol/mol) of PC to l-serine at 45°C within 40 min using 1.5 U PLD. The PLD could also synthesize PS from soybean lecithin with 100% conversion in the biphasic system at 1:7 (mol/mol) of soybean lecithin to l-serine at 45°C in 60 min. In addition, the PLDSC734 could be used to synthesize PS from soybean lecithin in an aqueous system as well with up to 94.7% conversion in the presence of 2.5 mM Triton X-100 in 100 min. Phospholipase D from Streptomyces sp. SC734 could be synthesized phosphatidylserine (PS) from phosphatidylcholine (PC) or lecithin and l-serine in both biphasic and aqueous systems.