Development of rapid assay for Schmallenberg virus by loop-mediated isothermal amplification method

Objective To set up loop-mediated isothermal amplification(RT-LAMP) method for quick detection of Schmallenbergvirus.Methods The S gene sequences of Schmallenbergvirus were downloaded from GenBank database and analyzed using MegAlign tools of software DNAStar.The outer primers and inner primers of LAMP were then designed according to their conservative region using software PrimerExplorer V4,and the reaction conditions were optimized.Results The results showed that the RTLAMP assay had a detection limit of 10 RNA copies.The primers used in this assay showed the specificity for Schmallenberg virus and did not amplify FMDV,BVDV,BTV,AKAV and VSV.Conclusion It is indicated the RT-LAMP method is a simple,rapid,accurate,sensitive and specific method for detecting Schmallenberg virus.This assay offers a new method and idea for detecting Schmallenberg virus. Key words: Schmallenberg virus;  Nucleic acid amplification techniques