Effect of recombinant granulocyte colony-stimulating factor on the chemiluminescence response of human polymorphonuclear leukocytes

The effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on the function of human polymorphonuclear leukocytes (PMNs), in terms of chemiluminescence (CL) response, were investigated in vitro. Incubation of rhG-CSF in concentrations of more than 4 ng/ml with PMNs obtained from healthy adults, at 37•Ž for 10 minutes, followed by stimulation with non-opsonized zymosan, significantly enhanced the CI, response of the PMNs, compared with the response in untreated PMNs. rhG-CSF exhibited a less powerful priming effect on the CL response of PMNs than recombinant human