Automated assay of N-acetyl-β-GLucosaminidase in normal and pathological human urine

Abstract An automated method for the assay of N -acetyl- β -glucosaminidase in human urine is described and a normal range of urinary N -acetyl- β -glucosaminidase activity is reported. The automated method has been used routinely over a twelve month period in two London hospitals for monitoring the excretion of N -acetyl- β -glucosaminidase in renal transplant patients. The abnormal elevation of urinary N -acetyl- β -glucosaminidase provides an early warning of rejection by indicating the presence of renal parenchymal damage. The automated assay of urinary N -acetyl- β -glucosaminidase may also be used in screening for the presence of renal disease.