Genetic and Genomic Manipulations in Aspergillus niger

Well-characterized selectable markers and promoters have been developed for the efficient transformation of Aspergillus niger for the production of proteins and for strain construction. Genetic transformation of this organism has been used widely for the production and secretion of homologous proteins and proteins from animals, plants, bacteria, and other fungi. The genome of A. niger harbors hundreds of genes encoding gene regulators, solute transporters, and biomass-degrading enzymes. Genome-wide and genome-based studies have substantially enhanced our understanding of the metabolic network, the roles of transcription regulators, and the identity of secondary metabolites. These studies have also led to the rational design of strains for the improved production and secretion of proteins and metabolites. With a finished genome sequence and robust transformation systems, A. niger is well positioned to become a synthetic biology platform.