Circulation time estimates of optically active nanoparticles using a pulse photometer
2009
Researchers employ increasingly complex sub-micron particles for oncological applications to deliver bioactive
therapeutic or imaging compounds to known and unknown in vivo tumor targets. In practice, experimental homogeneity
using nanoparticles can be difficult to achieve. While several imaging techniques have been previously shown to follow
the accumulation of nanoparticles into tumor targets, a more rapid sensor that provides a quantifiable estimate of dose
delivery and short-term systemic response could increase the clinical efficacy and greatly reduce the variability of these
treatments. We have developed a pulse photometer that when placed on an optically accessible location will estimate the
concentration of near-infrared absorbing nanoparticles. The goal is to monitor the accuracy of the delivered dose and the
effective circulation time of nanoparticles immediately after intravenous delivery but prior to therapeutic intervention.
We present initial tests of our prototype using murine models to assess its ability to quantify circulation half-life and
nanoparticle concentration. Four mice were injected with nanoparticles and circulation half-life estimates ranged from 3-
43 minutes. UV-Vis spectrophotometry was used to independently verify these measurements using 5μL blood samples.
Linear models relating the two methods produced R 2 values of 0.91, 0.99, 0.88, and 0.24.
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