DR3 (DRw17, DRw18): 2-D Gel Patterns

Six DR3 cell lines were included in the Workshop study (Table 1). Two-dimensional (2-D) gel patterns for both α chains and β chains were obtained using the panel of core monomorphic and selected polymorphic monoclonal antibodies. Each cell line was studied in two laboratories. The results obtained in different laboratories were generally concordant, and minor differences could be explained on the basis of differences in the efficiency of incorporation of labeled methionine. The complex pattern of class II β chains precipitated by the broadly reactive monomorphic antibodies could be dissected by using a combination of the results from the locus specific monomorphic and polymorphic antibodies. In this way, a specificity was assigned to each family of spots. In all six cell lines two distinct DRβ gene products could be identified, and a DQβ gene product was found in four of the cell lines.