Preparation of RNAII and validation of its virus inactivation

Purpose To purify RNAⅡ from calf pancreas and to validate the virus inactivationl by pasteurization.MethodsRNAⅡwas purified from pancreas by extraction of phenol and chloroform and RNA solution adding the porcine parvovirus(PPV)was heated to 60℃ for 10 hours.Purity,A260/A280,special UV absorbance peak and integrity were matched before and after pasteurization.ResultsHigh purity and activity of RNAⅡ were purified,and the initial titre of PPV added into RNAⅡ solution is 7.7logTCID50/0.1 mL.The residual virus was undetectable in the virus inactivation solution and the matched itoms didn't change qualitatively.ConclusionThe method of purification of RNAⅡ and inactivation of virus is effective.