Method and device for detecting nucleic acid analyte or by a total internal reflection technique.

SE provides an apparatus and method for detecting target nucleic acid EXPANDED IN WHICH THE PRESENCE AND CONCENTRATION OF DIANA IS EXPANDED determined by total internal reflection DURING THE COURSE OF THE REACTION AMPLIFICATION. ALSO it PROVIDES A method and apparatus for detecting target nucleic acid WHERE THE PRESENCE OF CONCENTRATION OF DIANA IS DETERMINED BY TOTAL INTERNAL REFLECTION AND COUPLING ELEMENT TO DIANA TIR cleavable by binding. It IS ALSO PROVIDES IMPROVED IMMUNOASSAY USING TOTAL REFLECTION AND CYCLE INTERNAL TEMPERATURE DIFFERENTIAL.