ROLE OF CALCIUM-PHOSPHATE BIONS IN PATHOGENESIS OF ATHEROSCLEROSIS: TOXICITY FOR ENDOTHELIUM

Aim. To assess the toxicity of calcium-phosphate bions (CPB) for epithelium in vitro and in vivo . Materials and methods. Artificial CPB synthesis, standard methods for cell culturing, phase-contrast microscopy, transmission electronic microscopy, annexin-V/7-aminoactinomycin D-test, 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT-test), immune-enzyme assay, original experimental model of rat aortal angioplasty using balloons for coronary angioplasty, hematoxylin-eosin stain and Van Gieson’s stain, histological analysis, statistical analysis were used. Results. CPB were internalized by endothelial cells of the EA.hy 926 line. The share of cells exposed to late apoptosis/necrosis was significantly higher, whereas, the share of bioplasts was significantly lower in the cultures of CPB-exposed cells as compared to the control cultures; no dose-dependence was revealed. The levels of interleukin-6 and interleukin-8 were significantly higher in the supernatant from CPB-exposed cells versus the control cultures. There was also observed a significant hyperplasia of intima in 3 CPB- exposed rats, but not in the controls. The intima to media ratio in the abdominal aorta wall was also significantly higher in CPB-exposed rates in comparison with the control rats. Conclusions. Calcium-phosphate bions have a toxic effect on endothelial cells.