Growth and invasion inhibitory effects of CBP gene on lung squamous carcinoma cell line NCI-H520

Objective:To investigate the effects of exogenous wild CSK-binding protein (CBP) gene stably transfection on growth and invastion of lung squamous carcinoma cells in vitro.Methods:A recombinant eukaryotic expression plasmid pcDNA3.0-CBP was constructed.Human lung cancer cell line NCI-H520 was transfected with pcDNA3.0-CBP or mock transfected plasmid pcDNA3.0(-) with lipofectamine2000,and cells stably expressing CBP were screened out by G418(800 mg /L).Changes of CBP protein and mRNA levels were measured by Western blotting and RT-PCR,cell viability was tested by MTT assay.Transwell and wound-healing methods were used to detect the difference of invasion and migration between transfected and non-transfected cells.Results:CBP protein and mRNA levels in pcDNA3.0-CBP transfected NCI-H520 cells were significantly higher than those in control NCI-H520 cells and pcDNA3.0(-) transfected NCI-H520 cells.The MTT assay revealed that the CBP gene transfected cells had less proliferative ability than control cells and pcDNA3.0(-) transfected NCI-H520 cells(both P 0.01).As compared with control cells and pcDNA3.0(-) transfected NCI-H520 cells,the invasion and migration ability of pcDNA3.0-CBP transfected cells decreased obviously (P 0.01).Conclusion:The CBP gene can restrain malignant phenotypes of the human lung squamous carcinoma in vitro and may participate in construction of negative feedback loop of SFKS to inhibit growth,migration and invasion of lung cancer cells.