Fractionation of venom from Vipera russelli siamensis and the effects of their components on blood coagulation

By gradient elution from DEAE-Sephadex A-50 column the venom of Vipera russelli siamensis (Smith) was separated into 8 fractions. Fraction II showed an antithromboplastin effect. Rechromatography of fractions V,VI,VIII and VII, all having procoagulant action,obtained 3 fractions from each. Fractions VI1,VI2 and VIII1 had stronger effects of procoagulant action than others; whereas fractions V2, VIII2 and VIII3 had antithrombin effects.