The synthetic chemotactic peptide formyl-methionyl-leucylphenylalanine causes an increase in actin associated with the cytoskeleton in rabbit neutrophils

Abstract Stimulation of rabbit peritoneal neutrophils by f-Met-Leu-Phe causes rapid and transient increase in the amount of cytoskeletal actin protein insoluble in 1% Triton X100. The amount of actin associated with the cytoskeleton exhibits a biphasic dose response curve reaching maximum association at 10 −9 M of f-Met-Leu-Phe. The stimulus-dependent increase in actin association with the cytoskeleton increases with time to reach a maximum value between 10 and 15 sec. after stimulation, followed by a reduction to control levels by 30 sec.