Genomic features of a polymyxin-resistant Klebsiella pneumoniae ST491 co-harbouring blaCTX-M-8 and qnrE1 genes isolated from a hospitalized cat in Sao Paulo, Brazil.

BACKGROUND: Klebsiella pneumoniae has emerged as one of the major pathogens to human and companion animals, whereas polymyxin resistance has begun to be increasingly reported worldwide, mainly among extended-spectrum beta-lactamase (ESBL) and/or carbapenemase producers. OBJECTIVE: To report the draft genome sequence of a polymyxin-resistant and extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae isolated from a domestic cat in Brazil. METHODS: The whole genome was sequenced on an Illumina NextSeq platform and de novo assembled using Velvet v. 1.2.10. Data analysis was performed using bioinformatics tools available from Center for Genomic Epidemiology and Pasteur database. RESULTS: The genome size was calculated at 5,260,459 bp, with a GC content of 57.3%, and comprising 5,294 total genes, 28 tRNAs, 7 rRNAs, 8 ncRNAs, and 237 pseudogenes. The K. pneumoniae 14CSI strain belonged to the sequence type ST491 presented a mutation (A14S) in the mgrB gene, and harboured blaCTX-M-8 and qnrE1 genes. Genes conferring resistance to heavy metals were further identified. CONCLUSION: This draft genome could be used as a reference sequence for comparative analysis of polymyxin-resistant and/or CTX-M-8-producing K. pneumoniae strains circulating at the human-animal interface.