[Effect of 2Me, DTT, ZAAP and Enzyme on JMH Antigen on the Surface of Human Erythrocytes].

题目: 2Me、DTT、ZAAP及酶等介质的处理对 红细胞JMH抗原的影响. 目的: 探讨血型鉴定、抗体鉴定及交叉配血过程中常用工具试剂2Me、DTT、木瓜蛋白酶、菠萝蛋白酶及ZZAP对人红细胞表面JMH抗原抗原性的影响. 方法: 首先分别用2-Me、DTT、菠萝蛋白酶、木瓜蛋白酶及ZZAP试剂对人红细胞进行处理，然后通过流式细胞术检测并分析处理前后人红细胞表面JMH抗原的变化. CONCLUSION: The treatment with 2-Me can reduce the antigenicity of JMH antigen on human RBC surface. The antigenicity of JMH antigen on human RBC surface increased after DTT treatment. The antigenicity of JMH antigen on human RBC surface significantly reduces after the treatment with pineapple protease or papain. ZZAP treatment can reduce the antigenicity of JMH antigen on the RBC surface. 结论: 2-Me处理可减弱人红细胞表面JMH抗原的抗原性；DTT处理后可增强人红细胞表面JMH抗原的抗原性；菠萝蛋白酶与木瓜蛋白酶的处理均可大幅度减弱人红细胞表面JMH抗原的抗原性；ZZAP可降低人红细胞表面JMH抗原的抗原性.