Pulse of dissolved organic matter alters reciprocal carbon subsidies between autotrophs and bacteria in stream food webs

Summary Soils are currently leaching out their organic matter at an increasing pace and darkening aquatic ecosystems due to climate and land use change, or recovery from acidification. The implications for stream biogeochemistry and food webs remain largely unknown, notably the metabolic balance (biotic CO2 emissions), reciprocal subsidies between autotrophs and bacteria, and trophic transfer efficiencies. We use a flow food web approach to test how a small addition of labile dissolved organic matter affects the strength and dynamics of the autotrophs-bacteria interaction in streams. Our paired streams whole-ecosystem experimental approach combined with continuous whole-stream metabolism and stable isotope probing allowed to unravel carbon fluxes in the control and treatment streams. We increased the natural supply of dissolved organic matter for three weeks by only 12% by continuously adding 0.5 mg L−1 of sucrose with a δ13C signature different from the natural organic matter. Both photosynthesis and heterotrophic respiration increased rapidly following C addition, but this was short lived due to N and P stoichiometric constraints. The resulting peak in heterotrophic respiration was of similar magnitude to natural peaks in the control observed when soils were hydrologically connected to the streams and received soil derived carbon. Carbon reciprocal subsidies between autotrophs and bacteria in the control stream accounted for about 50% of net primary production and 75% of bacterial production, under low flow conditions when stream water was hydrologically disconnected from soil water. The reciprocal subsidies were weaker by 33% (autotrophs to bacteria) and 55% (bacteria to autotrophs) in the treatment relative to the control. Net primary production relied partly (11% in the control) on natural allochthonous dissolved organic carbon via the CO2 produced by bacterial respiration. Many large changes in ecosystem processes were observed in response to the sucrose addition. The light use efficiency of the autotrophs increased by 37%. Ecosystem respiration intensified by 70%, and the metabolic balance became relatively more negative, i.e. biotic CO2 emissions increased by 125%. Heterotrophic respiration and production increased by 89%, and this was reflected by a shorter (−40%) uptake length (SwOC) and faster (+92%) mineralisation velocity of organic carbon. The proportion of DOC flux respired and organic carbon use efficiency by bacteria increased by 112%. Macroinvertebrate consumer density increased by 72% due to sucrose addition and consumer production was 1.8 times higher in the treatment than in the control at the end of the experiment. The trophic transfer efficiencies from resources to consumers were similar between the control and the treatment (2-5%). Synthesis. Part of the carbon derived from natural allochthonous organic matter can feed the autotrophs via the CO2 produced by stream bacterial respiration, intermingling the green and brown webs. The interaction between autotrophs and bacteria shifted from mutualism to competition with carbon addition under nutrient limitation (N, P) increasing biotic CO2 emissions. Without nutrient limitation, mutualism could be reinforced by a positive feedback loop, maintaining the same biotic CO2 emissions. A small increase in dissolved organic carbon supply from climate and land use change could have large effects on stream food web and biogeochemistry with implications for the global C cycle under stoichiometric constraints.