Stabilizing the urinary activity of fructose-1,6-bisphosphatase with EDTA and mercaptoethanol.

Abstract Objectives The study aim was to establish conditions for stabilization the activity of fructose-1,6-bisphosphatase (FBP-1) in stored urine. Design and methods The FBP-1 was determined by the method Kepka et. al in a collected fraction of purified urine. Results EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine. Conclusion At optimal conditions urine may be stored up to 7 days at a temperature of 4 °C.