Novel fluorescent probe, preparation method thereof and application of novel fluorescent probe to detection of 6-thioguanine

The invention discloses a novel fluorescent probe, a preparation method thereof and application of the novel fluorescent probe to detection of 6-thioguanine (6-TG). The preparation method comprises the following steps: taking DTPA (Diethylene Triamine Pentoacetic Acid), acetic anhydride and pyridine, slowly stirring and heating at the temperature of 65 DEG C for 24 hours; cooling to room temperature, filtering, washing with anhydrous ether, performing suction filtration, and performing vacuum drying to obtain DTPAA (Diethylenetriaminpentacetic Acid); taking the DTPAA, triethylamine, DMF (Dimethyl Formamide) and cytosine, keeping the temperature at 100 DEG C, heating and stirring for 24 hours; cooling to the room temperature, filtering, and performing vacuum drying to obtain DTPA-bis(cytosine); taking the DTPA-bis(cytosine), dissolving the DTPA-bis(cytosine) in a Tris-HCl buffer solution of which the pH is equal to 7.4, washing Eu(NO3)3.6H2O with the solution, collecting washing liquid, and heating or standing for a long time to obtain the fluorescent probe. When the fluorescent probe is applied to the detection of the 6-TG together with a fluorescence method, a simple and novel method is provided, high efficiency is achieved, the cost is low, and environmental pollution is avoided.