A field of rapid detection of Brucella universal primers and probes and kits for

The present invention discloses a universal primers and probes and a kit for on-site rapid detection of Brucella, the forward primer sequences as shown SEQIDNo.1, reverse primer sequence as shown SEQIDNo.2, EXPLORATION as shown in the sequence SEQIDNo.3 needle. The present invention detects Brucella high sensitivity and specificity, can detect a minimum of 6.0 × 100cfu Brucella, Escherichia coli O157: H7, Yersinia enterocolitica O: 9, Salmonella, Staphylococcus aureus aureus and other bacteria no cross reaction. The present invention is applicable not only to detect strain of Brucella can be used for detection of clinical samples. Clinical samples including blood and milk samples, aerosol samples, tissue samples and so on. The kit of the present invention is easy to use without special equipment, only at 38 ℃, 25min treatment can be measured for samples of the crude lysate Brucella sensitive, specific and rapid detection of suitable site or substrate fabric sick and quarantine work.